Optimization of Effective Parameters on Biohydrogen Production Using Bioaugmentation

Document Type : Original Research

Authors

Chemical Engineering Department, Faculty of Engineering, Shahid Bahonar University of Kerman, Iran.

Abstract

Biohydrogen production is a green method that utilizes organic materials in activated sludge as a substrate. The process involves microorganisms contracting the volume of the sludge content to produce hydrogen, CO2, CH4, and etc. However, the efficiency of this process is low. In this study, bioaugmentation was carried out by inoculating a 10% Escherichia coli suspension adapted in whey and activated sludge medium. The effects of parameters such as pH, temperature, and stirring, the concentration of whey as carbon source and nitrate as nitrogen source on hydrogen production were screened using the Plackett-Burman method with Minitab 21 software. Among the selected parameters, pH, temperature, concentration of whey and nitrate were found to be the most effective parameters in hydrogen production and were further optimized using Response surface methodology. Stirring wasn’t statistically significant. The optimum conditions for hydrogen production were pH=5.4, temperature=39 ˚C, whey concentration=30 g/L, and nitrate concentration=3.6 g/L. Under these conditions with a 10% inoculation, the total volume of gas production was extended to 1.61 L per liters of activated sludge with 0.046 mole H2 per liters of activated sludge. Comparing the bioaugmentation method with other method showed that the total time of the process decreased by 8 hours. Additionally, hydrogen production started after 10 hours of incubation and reached its maximum value in 16 hours, resulting in a 59% increase in productivity in less than 16 hours.

Keywords

References

Abendroth, C., Vilanova, C., Günther, T., Luschnig, O., & Porcar, M. (2015). Eubacteria and archaea communities in seven mesophile anaerobic digester plants in Germany. Biotechnology for Biofuels, 8, 1-10. https://doi.org/https://doi.org/10.1186/s13068-015-0271-6
Argun, H., & Kargi, F. (2011). Bio-hydrogen production by different operational modes of dark and photo-fermentation: an overview. International Journal of Hydrogen Energy, 36(13), 7443-7459. https://doi.org/https://doi.org/10.1016/j.ijhydene.2011.03.116
Bao, M., Su, H., & Tan, T. (2013). Dark fermentative bio-hydrogen production: Effects of substrate pre-treatment and addition of metal ions or L-cysteine. Fuel, 112, 38-44. https://doi.org/https://doi.org/10.1016/j.fuel.2013.04.063
Baskaran, S., & Sathiavelu, M. (2022). Bioaugmentation and biostimulation of dumpsites for plastic degradation. In Cost Effective Technologies for Solid Waste and Wastewater Treatment (pp. 9-23). Elsevier. https://doi.org/https://doi.org/10.1016/B978-0-12-822933-0.00015-2
Choi, Y., Ryu, J., & Lee, S. R. (2020). Influence of carbon type and carbon to nitrogen ratio on the biochemical methane potential, pH, and ammonia nitrogen in anaerobic digestion. Journal of animal science and technology, 62(1), 74. https://doi.org/https://doi.org/10.5187/jast.2020.62.1.74
Feldewert, C., Lang, K., & Brune, A. (2020). The hydrogen threshold of obligately methyl-reducing methanogens. FEMS Microbiology Letters, 367(17), fnaa137. https://doi.org/https://doi.org/10.1093/femsle/fnaa137
Gao, Y., Cai, M., Shi, K., Sun, R., Liu, S., Li, Q., . . . Xue, J. (2023). Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways. Water Science & Technology, 87(1), 228-238. https://doi.org/https://doi.org/10.2166/wst.2022.406
Ghimire, A., Frunzo, L., Pirozzi, F., Trably, E., Escudie, R., Lens, P. N. L., & Esposito, G. (2015). A review on dark fermentative biohydrogen production from organic biomass: process parameters and use of by-products. Applied Energy, 144, 73-95. https://doi.org/https://doi.org/10.1016/j.apenergy.2015.01.045
Gokulapriya, G., Chandrasekaran, M., & Soundararajan, R. (2022). Bioaugmentation of Pesticides-Contaminated Environment. In Bioaugmentation Techniques and Applications in Remediation (pp. 29-42). CRC Press. https://doi.org/https://doi.org/10.1201/9781003187622-3
Hassa, J., Maus, I., Off, S., Pühler, A., Scherer, P., Klocke, M., & Schlüter, A. (2018). Metagenome, metatranscriptome, and metaproteome approaches unraveled compositions and functional relationships of microbial communities residing in biogas plants. Applied microbiology and biotechnology, 102, 5045-5063. https://doi.org/https://doi.org/10.1007/s00253-018-8976-7
Rashama, C., Ijoma, G. N., & Matambo, T. S. (2022). Elucidating biodegradation kinetics and biomethane potential trends in substrates containing high levels of phytochemicals: the case of avocado oil processing by-products. Waste and Biomass Valorization, 13(4), 2071-2081.https://doi.org/10.1007/s12649-021-01663-z
 
Karadag, D., Köroğlu, O. E., Ozkaya, B., Cakmakci, M., Heaven, S., & Banks, C. (2014). A review on fermentative hydrogen production from dairy industry wastewater. Journal of Chemical Technology & Biotechnology, 89(11), 1627-1636. https://doi.org/10.1002/jctb.4490
Kaza, S., Yao, L., Bhada-Tata, P., & Van Woerden, F. (2018). What a waste 2.0: a global snapshot of solid waste management to 2050. World Bank Publications. https://doi.org/https://doi.org/10.1596/978-1-4648-1329-0
Khesareh, S., & Ataei, S. A. (2023). Regulation of Metabolic Pathway for Bio-Hydrogen Production in Dark Fermentation via Redox Potential. Biomechanism and Bioenergy Research, 2(2), 10-18. https://doi.org/https://doi.org/10.22103/BBR.2023.22231.1054
Lee, M.-J., Zhang, S., Cho, Y.-B., Park, J.-E., Chang, K.-H., & Hwang, S.-J. (2015). Effects of nitrate concentration on biohydrogen production and substrate utilization in dark-fermentation. Journal of Material Cycles and Waste Management, 17, 27-32. https://doi.org/https://doi.org/10.1007/s10163-013-0219-5
Mazzurco Miritana, V., Gaetani, A., Signorini, A., Marone, A., & Massini, G. (2023). Bioaugmentation strategies for enhancing methane production from shrimp processing waste through anaerobic digestion. Fermentation, 9(4), 401. https://doi.org/https://doi.org/10.3390/fermentation9040401
Mishra, P., Krishnan, S., Rana, S., Singh, L., Sakinah, M., & Ab Wahid, Z. (2019). Outlook of fermentative hydrogen production techniques: An overview of dark, photo and integrated dark-photo fermentative approach to biomass. Energy Strategy Reviews, 24, 27-37. https://doi.org/https://doi.org/10.1016/j.esr.2019.01.001
Mu, Y., Zheng, X.-J., Yu, H.-Q., & Zhu, R.-F. (2006). Biological hydrogen production by anaerobic sludge at various temperatures. International Journal of Hydrogen Energy, 31(6), 780-785. https://doi.org/https://doi.org/10.1016/j.ijhydene.2005.06.016
Okonkwo, O., Escudie, R., Bernet, N., Mangayil, R., Lakaniemi, A.-M., & Trably, E. (2020). Bioaugmentation enhances dark fermentative hydrogen production in cultures exposed to short-term temperature fluctuations. Applied microbiology and biotechnology, 104, 439-449. https://doi.org/https://doi.org/10.1007/s00253-019-10203-8
Pugazhendhi, A., Shobana, S., Nguyen, D. D., Banu, J. R., Sivagurunathan, P., Chang, S. W., . . . Kumar, G. (2019). Application of nanotechnology (nanoparticles) in dark fermentative hydrogen production. International Journal of Hydrogen Energy, 44(3), 1431-1440. https://doi.org/https://doi.org/10.1016/j.ijhydene.2018.11.114
Sharma, S., Basu, S., Shetti, N. P., & Aminabhavi, T. M. (2020). Waste-to-energy nexus for circular economy and environmental protection: Recent trends in hydrogen energy. Science of the Total Environment, 713, 136633. https://doi.org/https://doi.org/10.1016/j.scitotenv.2020.136633
Shaterzadeh, M. J., & Ataei, S. A. (2017). The effects of temperature, initial pH, and glucose concentration on biohydrogen production from Clostridium acetobutylicum. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 39(11), 1118-1123. https://doi.org/https://doi.org/10.1080/15567036.2017.1297875
Sheng, T., Meng, Q., Wen, X., Sun, C., Yang, L., & Li, L. (2021). Bioaugmentation with Ruminiclostridium thermocellum M3 to enhance thermophilic hydrogen production from agricultural solid waste. Journal of Chemical Technology & Biotechnology, 96(6), 1623-1631. https://doi.org/https://doi.org/10.1002/jctb.6682
Silva, J., Mendes, J., Correia, J., Rocha, M., & Micoli, L. (2018). Cashew apple bagasse as new feedstock for the hydrogen production using dark fermentation process. Journal of biotechnology, 286, 71-78. https://doi.org/https://doi.org/10.1016/j.jbiotec.2018.09.004
Srivastava, N., Srivastava, M., Malhotra, B. D., Gupta, V. K., Ramteke, P. W., Silva, R. N., . . . Mishra, P. K. (2019). Nanoengineered cellulosic biohydrogen production via dark fermentation: a novel approach. Biotechnology advances, 37(6), 107384. https://doi.org/https://doi.org/10.1016/j.biotechadv.2019.04.006
Stolze, Y., Bremges, A., Rumming, M., Henke, C., Maus, I., Pühler, A., . . . Schlüter, A. (2016). Identification and genome reconstruction of abundant distinct taxa in microbiomes from one thermophilic and three mesophilic production-scale biogas plants. Biotechnology for Biofuels, 9, 1-18. https://doi.org/https://doi.org/10.1186/s13068-016-0565-3
Venkiteshwaran, K., Bocher, B., Maki, J., & Zitomer, D. (2015). Relating anaerobic digestion microbial community and process function: supplementary issue: water microbiology. Microbiology insights, 8. https://doi.org/https://doi.org/10.4137/MBI.S33593
Wang, J., & Yin, Y. (2019). Progress in microbiology for fermentative hydrogen production from organic wastes. Critical reviews in environmental science and technology, 49(10), 825-865. https://doi.org/https://doi.org/10.1080/10643389.2018.1487226
Wang, Z.-H., Tan, J.-Y., Zhang, Y.-T., Ren, N.-Q., & Zhao, L. (2022). Evaluating bio-hydrogen production potential and energy conversion efficiency from glucose and xylose under diverse concentrations. Fermentation, 8(12), 739. https://doi.org/https://doi.org/10.3390/fermentation8120739
Zhang, L., Tian, H., Lee, J. T., Lim, J. W., Loh, K.-C., Dai, Y., & Tong, Y. W. (2022). Bioaugmentation strategies via acclimatized microbial consortia for bioenergy production. In Biomass, Biofuels, Biochemicals (pp. 179-214). Elsevier. https://doi.org/https://doi.org/10.1016/B978-0-323-90633-3.00018-3
Zhang, Y., Zhang, H., Lee, D.-J., Zhang, T., Jiang, D., Zhang, Z., & Zhang, Q. (2020). Effect of enzymolysis time on biohydrogen production from photo-fermentation by using various energy grasses as substrates. Bioresource Technology, 305, 123062. https://doi.org/https://doi.org/10.1016/j.biortech.2020.123062
Biomechanism and Bioenergy Research
Volume 3, Issue 1
June 2024
Pages 68-80

Files

History

  • Receive Date: 02 April 2024
  • Revise Date: 17 May 2024
  • Accept Date: 26 May 2024

Share

How to cite

Statistics